Textbook

Overview

Introduction to Optical Microscopy provides a rigorous and comprehensive overview of the fundamentals of optical microscopy. The book begins with the basic principles in Fourier optics, partial coherence, 3D imaging theory, and the physics of scattering and fluorescence.

Introduction to Optical Microscopy explores a broad range of microscopy techniques. These include classical techniques such as phase contrast, confocal microscopy, etc., and progress to more modern techniques such as holographic microscopy, optical coherence tomography, two-photon microscopy, coherent anti-Stokes Raman scattering microscopy, etc.

The final chapters present a survey of new directions, including structured illumination and superresolution. Introduction to Optical Microscopy is designed to provide a solid theoretical foundation for graduate students or researchers who want to enter the field. While valuable as a reference, it can also serve as a textbook, featuring an updated website that provides problem sets and an instructor's solution manual.

Table of Contents

  1. Introduction
  2. Monochromatic Wave Propagation
  3. Monochromatic Field Propagation through a Lens
  4. Intensity Propagation
  5. 3D Imaging
  6. Radiometery
  7. Intensity Fluctuations
  8. Detection Noise
  9. Absorption and Scattering
  10. Phase Contrast
  11. Holographic Microscopy
  12. Optical Coherence Tomography
  13. Fluorescence
  14. Confocal Microscopy
  15. Two-Photon Microscopy
  16. Coherent Nonlinear Microscopy
  17. Pupil Synthesis
  18. Superresolution

Additional Resources